In-depth Study of the Biodiversity of the Fungus Causing Olive Tree Anthracnose

The Department of Agronomy at the University of Córdoba leads an international study in which the different species of the pathogen are being understood thanks to samples collected over the course of 25 years.

The olive tree is one of the most important woody crops in the world, its cultivated area occupying more than 11 million hectares (FAO, 2019). Due to its economic, social and cultural importance, study of the pathogens that affect the tree and its fruit is crucial to find control methods that prevent damage.


Among the foliar diseases that affect the olive tree, anthracnose is considered one of the most important. This disease, popularly known as cankers, is caused by species of fungi of the genus Colletotrichum, and causes high losses on fruit yield and oil quality during epidemic years.


Thus, a team from the María de Maeztu Unit of Excellence—Department of Agronomy at the University of Córdoba (DAUCO) has conducted an international study featuring the specific characterization of a collection of 185 Colletotrichum isolates (samples extracted from olive trees with symptoms of anthracnose and containing the pure organism) from various enclaves around the world.


Previously, only two complex species of the genus Colletotrichum were known to cause olive anthracnose, but, after various taxonomic revisions, up to 18 species of the fungus associated with the disease have been distinguished worldwide. With the aim of obtaining more in-depth knowledge of the biology of the pathogen and, thus, being able to select the most effective control measures against it, this DAUCO team has characterized the collection of Colletotrichum isolates obtained over 25 years of work, and with samples not only from Spain, but also from Australia, Brazil, California, Greece, Italy, Portugal, Tunisia and Uruguay; as well as other hosts susceptible to the disease, such as almonds, strawberries, oranges and pistachios.


In the study, led by researchers from the Department of Agronomy at the UCO Juan Moral, Carlos Agustí and Antonio Trapero "a phenotypic, molecular and pathogenic characterization has been carried out, molecular characterization being the decisive one for the identification of the species," explained Antonio Trapero. "In the case of Colletotrichum, its morphological characteristics do not allow us to differentiate between different species, so we must turn to DNA sequences that tell us how similar some isolates are to others," adds Juan Moral, in order to be able to classify them by phylogenetic species.


After using 7 specific gene regions for this identification, 12 species of Colletotrichum were identified in the collection studied, establishing the most widespread species in Spain, Greece, Tunisia, Portugal and California. In these producing areas there is usually one dominant species and one or two more secondary ones. The highest species diversity was found in Australia, with 8 different Colletotrichum species. More


Read the entire Report Diversity of Colletotrichum Species Associated with Olive Anthracnose Worldwide here